The predetermination of embryonic sex using flow cytometrically separated X and Y spermatozoa
نویسندگان
چکیده
منابع مشابه
Application and commercialization of flow cytometrically sex-sorted semen.
The current technology to sort X and Y chromosome bearing sperm population requires individual identification and selection of spermatozoa in a modified high-speed flow cytometer. For farm animal species, the technology is capable of producing sexed sperm at greater than 90% purity. However, only in the bovine, the technology has reached a developmental level that allows its commercial applicat...
متن کاملP-101: Study of The Effect of Alkaline Medium on Separation of Spermatozoa Containing X and Y Chromosomes in order to Sex Selection
Background: Sex Selection in livestock happens in order to increase productivity and it also occurs in human society to avoid genetic disease related to gender and culture, social and religious trends. By separating spermatozoa carrying X and Y chromosomes from each other, we could achieve a desire gender by using assisted productive techniques. In this study the effect of alkaline environment ...
متن کاملon the relationship between using discourse markers and the quality of expository and argumentative academic writing of iranian english majors
the aim of the present study was to investigate the frequency and the type of discourse markers used in the argumentative and expository writings of iranian efl learners and the differences between these text features in the two essay genres. the study also aimed at examining the influence of the use of discourse markers on the participants’ writing quality. to this end the discourse markers us...
15 صفحه اولVerification of flow cytometorically-sorted X- and Y-bearing porcine spermatozoa and reanalysis of spermatozoa for DNA content using the fluorescence in situ hybridization (FISH) technique.
Flow cytometric sperm sorting based on X and Y sperm DNA difference has been established as the only effective method for sexing the spermatozoa of mammals. The standard method for verifying the purity of sorted X and Y spermatozoa has been to reanalyze sorted sperm aliquots. We verified the purity of flow-sorted porcine X and Y spermatozoa and accuracy of DNA reanalysis by fluorescence in situ...
متن کاملEvaluation of Discontinues Puresperm Gradient in Separation of X And Y Bearing Human Spermatozoa by Fluorescent in Situ Hybridization (FISH)
Introduction: To evaluate the 8 layer discontinues puresperm gradient in separation of human spermatozoa according to sex chromosomes by fluorescent in situ hybridization (FISH). Material and Methods: This study was carried out on three patient referring to Royan fertility and infertility center. Semen analysis was assessed according to WHO criteria. Each sample was divided into two aliquots, ...
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ژورنال
عنوان ژورنال: Human Reproduction Update
سال: 1996
ISSN: 1355-4786,1460-2369
DOI: 10.1093/humupd/2.4.355